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產(chǎn)品名稱：（非衍生化 串聯(lián)質(zhì)譜法LC-MS/MS ）

產(chǎn)品型號：57000

產(chǎn)品報價：

產(chǎn)品特點：

供應(yīng)商：祥生興業(yè)規(guī)格：960analyses新生兒遺傳代謝疾病檢測

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57000（非衍生化 串聯(lián)質(zhì)譜法LC-MS/MS ）的詳細資料：

溫馨提示：僅用于科研，不可用于臨床治療

  • 供應(yīng)商：

    祥生興業(yè)

  • 規(guī)格：

    960 analyses

    新生兒遺傳代謝疾病檢測，串聯(lián)質(zhì)譜分析方法試劑盒LC-MS/MS 非衍生化

    • All required and isotope labelled standards included

    • Dried blood spot controls included

    • Direct sample injection without derivatisation

    • High selectivity and sensitivity by MS/MS detection

    This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hａnd lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.

    Specifications

    Linearity: Amino acids: 2000-2400 µmol/l
    (Acyl-)carnitines: 25-200 µmol/l
    Limit of quantification: Amino acids: 2.5-30 µmol/l
    (Acyl-)carnitines: 0.1-1.6 µmol/l
    Recovery: Amino acids: 64-94 %
    (Acyl-)carnitines: 63-102 %
    Intra-assay: Amino acids: 2-11 %
    (Acyl-)carnitines: 3-12 %
    Inter-assay: Amino acids: 3.5-18 %
    (Acyl-)carnitines: 4.8-14 %
    Analysis time: < 2 min

    Cutoff thresholds

    Amino acids: ca. 30 to 1000 µmol/l
    free carnitine: ca. 55 µmol/l
    Acylcarnitines: ca. 0.2 to 10 µmol/l

    LC-MS/MS parameters

    LC-MS/MS system. No HPLC column is required.

    Injection volume: 10 µl
    Flow gradient: 20 to 600 µl/min
    Autosampler rinsing solution: Rinsing Solution (57007)
    MS/MS Mode: MRM
    Mass transitions:
    Alanine 90 > 44
    Alanine-D4 94 > 48
    Arginine 175 > 70
    Arginine-D7 182 > 77
    Aspartic acid 134 > 116
    Aspartic acid-D3 137 > 119
    Citrulline 176 > 113
    Citrulline-D2 178 > 115
    Glutamic acid 148 > 130
    Glutamic acid-D5 153 > 135
    Glycine 76 > 30
    Glycine-13C2-15N 79 > 32
    Leucine 132 > 86
    Leucine-D3 135 > 89
    Methionine 150 > 133
    Methionine-D3 153 > 136
    Ornithine 133 > 70
    Ornithine-D6 139 > 76
    Phenylalanine 166 > 120
    Phenylanlanine-D5 171 > 125
    Proline 116 > 70
    Proline-D7 123 > 77
    Tyrosine 182 > 136
    Tyrosine-D4 186 > 140
    Valine 118 > 72
    Valine-D8 126 > 80
    Free carnitine 162 >85
    Carnitine-D9 171 > 85
    C2-Carnitine 204 > 85
    C2-Carnitine-D3 207 > 85
    C3-Carnitine 218 > 85
    C3-Carnitine-D3 221 > 85
    C4-Carnitine 232 > 85
    C4-Carnitine-D3 235 > 85
    C5-Carnitine 246 > 85
    C5-Carnitine-D9 255 > 85
    C5DC-Carnitine 276 > 85
    C5DC-Carnitine-D6 282 > 85
    C6-Carnitine 260 > 85
    C6-Carnitine-D3 263 > 85
    C8-Carnitine 288 > 85
    C8-Carnitine-D3 291 > 85
    C10-Carnitine 316 > 85
    C10-Carnitine-D3 319 > 85
    C12-Carnitine 344 > 85
    C12-Carnitine-D3 347 > 85
    C14-Carnitine 372 > 85
    C14-Carnitine-D3 375 > 85
    C16-Carnitine 400 > 85
    C16-Carnitine-D3 403 > 85
    C18-Carnitine 428 > 85
    C18-Carnitine-D3 431 > 85

    Sample preparation

    • Punch 3 mm dried blood spot disk out of the filter card into a well plate.
    • Add 100 µl Internal Standard, agitate for 20 min.
    • Inject 10 µl of the extract into the LC-MS/MS system.

    Order no. Product
    57000 MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with regular 96 well plates.
    For 960 analyses.
    57000/F MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with 96 well filter plates.
    For 960 analyses.
    Separately available components
    57001 Mobile Phase, 1000 ml
    57002 Mobile Phase, 10 x 1000 ml
    57004/T Internal Standard (lyoph.), 25 ml
    57004 Internal Standard (lyoph.), 4 x 25 ml
    57007 Rinsing Solution, 1000 ml
    57008 Extraction Buffer, 100 ml
    57010 96-Well-Plates, V-Boden, 5 pcs.
    55011 Protective sheets for 96 well plates, 10 pcs.
    57057 96 Well filter plates, 5 pcs.
    55013 Piercable adhesive seal for 96 well plates, 10 pcs.
    Startup accessories
    55015 Restrictor Capillary, 1 pc.
    55016 Adapter collar for centrifugation, 2 pcs.
    57099 Tuning Mix, incl. all analytes, 2 ml
    55033 PEEK prefilter, 5 µm, 5 pcs.
    15010 PEEK prefilter housing, 1 pc.
    Chromsystems quality control material (filter spot)
    0191 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots
    0192 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots
    0193 MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots

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