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產(chǎn)品名稱：變腎上腺檢測試劑盒LC-MS/MS

產(chǎn)品型號：81000 81000/C

產(chǎn)品報價：

產(chǎn)品特點：

英文名：MassChrom®FreeMetanephrinesinPlasmawith96SPEWellPlate供應(yīng)商：祥生興業(yè)規(guī)格：2x96TestsMassChrom®FreeMetanephrinesinPlasmawith96SPEWellPlateOrderno

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81000 81000/C變腎上腺檢測試劑盒LC-MS/MS的詳細資料：

溫馨提示：僅用于科研，不可用于臨床治療

  • 英文名：

    MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate

  • 供應(yīng)商：

    祥生興業(yè)

  • 規(guī)格：

    2 x 96 Tests

    MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate


    Order no.: 81000
    2 x 96 Tests
    • 3-Methoxytyramine
    • Metanephrine
    • Normetanephrine

    6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma

    Isotopically labelled internal standard for each analyte

    Fully automated workflow solution available
     

    DESCRIPTION

    MassChrom® Free Metanephrines in Plasma
    With 96 SPE Well Plates, suitable for manual and automated sample preparation
    For 2 x 96 determinations

     

    Clinical relevance

    This kit allows the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.

    One typical application for this kit is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.


     

    Product advantages

    • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
    • Simple sample prep in just a few steps
    • Chromatographic separation of analytes – no co-elution
    • Isotopically labelled internal standards for all analytes
    • Fully automated workflow solution available

     

    This kit encompasses all required components and allows a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1® Multilevel Plasma Calibrator Set and MassCheck® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.

    A fully automated workflow solution is also available.

    Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).

    CHROMATOGRAM

    EN

    TECHNICAL DATA

    Method of Analysis

    LC-MS/MS

    Number of Tests

    2 x 96

    Parameter

    3-Methoxytyramine, Metanephrine, Normetanephrine

    Analysis Time

    5 min

    Limit of quantification

    Metanephrine: 15 ng/l
    Normetanephrine: 7 ng/l 
    3-Methoxytyramine: 11 ng/l

    Linearity

    Metanephrine: up to 5650 ng/l
    Normetanephrine: up to 8500 ng/l 
    3-Methoxytyramine: up to 5800 ng/l

    Recovery

    89–100 %

    Intraassay

    CV = 2.7–6.9 %

    Interassay

    CV = 3.3–8.6 %

    Gradient

    Binary

    Specimen

    Plasma

    Sample Preparation

    • Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each well of the 96 SPE Well Plate.
    • Add 500 µl of the sample/calibrator/MassCheck® control, centrifuge, discard effluent.
    • Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
    • Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
    • Add 250 µl Elution Buffer into each well and centrifuge.
    • Inject up to 25 µl.

    Injection Volume

    ≤ 25 µl

    Ionisation

    ESI positive

    MS/MS-Mode

    MRM

    Please note

    The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

    KIT COMPONENTS

    Components available separately

    Mobile Phase A

    Order no.: 81001

    DETAILS

    Mobile Phase B

    Order no.: 81002

    DETAILS

    Dilution Buffer

    Order no.: 81003

    DETAILS

    Internal Standard Mix

    Order no.: 81004

    DETAILS

    Wash Buffer 1

    Order no.: 81005

    DETAILS

    Wash Buffer 2

    Order no.: 81006

    DETAILS

    Elution Buffer

    Order no.: 81007

    DETAILS

    Rinsing Solution

    Order no.: 81009

    DETAILS

    96 SPE Well Plate

    Order no.: 81057

    DETAILS

    Collection Plates

    Order no.: 81058

    DETAILS

    Pierceable adhesive seals for 96 Well Plates

    Order no.: 81059

    DETAILS

    ACCESSORIES

    Analytical Column

    Order no.: 81100

    DETAILS

    Tuning Mix Free Metanephrines in Plasma

    Order no.: 81015

    DETAILS

    System Check Solution

    Order no.: 81010

    DETAILS

    Waste Plates

    Order no.: 81056

    DETAILS

    Stainless steel prefilter housing

    Order no.: 15070

    DETAILS

    Stainless steel prefilter - 0.5 µm

    Order no.: 15071

    DETAILS

    CALIBRATORS

    Calibrators available separately

    6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines

    Order no.: 81039

    DETAILS

    CONTROLS

    Controls available separately

    MassCheck® Free Metanephrines Plasma Controls

     

    DETAILS





     

溫馨提示：不可用于臨床治療。
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